Oxion Fluorescence

Fluorescensmikroskop för biologi och medicin som används för att synliggöra specifika delar av levande celler och vävnader med hjälp av fluorofores. En eller flera fluoforer tillsätts provet som ska undersökas. Då fluoroforesförberedda prover utsätts för så kallat excitationsljus så absorberas excitationsenergin av fluoroforerna och de börjar avge så kallat utstrålningsljus som man kan se med mikroskopet. Med denna teknik kan tumörceller urskiljas bland vanliga celler, biologiska processer kan synliggöras och följas samt så kan närvaro eller frånvaro av antikroppar urskiljas, för att nämna några applikationer.

The Oxion microscopes for fluorescence are equipped with an epi-illumination with a 100 W mercury vapor lamp. The fluorescence attachment have a rail for maximum 4 filter cubes. The FluoLed model is equipped with a 455 nm LED and one filter cube, especially developed for efficient and fast detection of tuberculosis by the Auramine-O fluorochrome. Other FluoLED wavelengths are also available

EYEPIECE(S)
HWF 10x/22 mm eyepieces. All eyepieces can be secured by an Allen screw

HEADS
Binocular and trinocular Siedentopf type heads are with 30° inclined tubes. Interpupillary distance from 50 to 75 mm. Two ± 5 diopter adjustments. A unique rotating system allows the ergonomical positioning of both tubes in a high (431 mm) and in a low position (397 mm)

NOSEPIECE
Revolving and reversed nosepiece for maximum 5 objectives. The fluorescence models with mercury vapor lamp are equipped with an orange UV protection shield

OBJECTIVES
• Plan 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion IOS objectives
• Plan fluarex 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion IOS objectives
• Plan semi apochromatic fluarex 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion IOS objectives
All S40x, S60x and S100x oil immersion objectives are spring loaded. All optics are anti-fungus treated and anti-reflection coated for maximum light throughput

FOCUSING
Coaxial coarse and fine adjustments, 200 graduations,1 μm per graduation, 200 μm per rotation, total travel approximately 28 mm. Supplied with an adjustable rack stop to prevent damage to sample and objectives. The coarse adjustments are equipped with friction control

STAGE
150 x 140 mm stage with integrated X-Y mechanical stage. A ceramic stage is also available on request

CONDENSER FOR BRIGHTFIELD
Height adjustable Abbe condenser N.A. 1.25 with iris diaphragm. Supplied with a white diffusion filter. Phase contrast rings mounted in slider can be inserted in the condenser for 10x and S40x or 20x and S100x phase contrast objectives. A dark field stop in slider for the 10x, 20x and S40x objectives can also be inserted with magnification indication

CONDENSER FOR FLUORESCENCE
The fluorescence attachment is equipped with an integrated condenser with iris diaphragm and with a field diaphragm

ILLUMINATION FOR BRIGHTFIELD AND PHASE CONTRAST
All models are supplied with a 3 W adjustable NeoLED illumination with built-in 100-240 V power supply. Köhler illumination with field diaphragm

ILLUMINATION FOR FLUORESCENCE
Fluorescence attachment with 100 W (HBO) mercury vapor incident epi-illumination and rail for maximum 4 filter blocks. Two general purpose filter sets are standard supplied with the attachment: AE.3245 (Blue excitation) and AE.3246 (Green excitation). Two other general purpose filter sets AE.3246 (UV excitation) and AE.3247 (Ultraviolet excitation) are also available. More specific filter sets are available on request

ILLUMINATION FOR FLUOLED ATTACHMENT
The FluoLed attachment is standard delivered with a 455 nm LED for efficient and fast detection of tuberculosis with Auramine-O fluorochrome. FluoLED attachments are also available with a 560 nm LED and Green filter set or a 365 nm LED together with an Ultraviolet filter set

PACKAGING
Supplied with power cord, dust cover, white filter, user manual and 5 ml immersion oil for models with S100x objective. All packed in a polystyrene box