iScope fluorescence

Fluorescensmikroskop för biologi och medicin som används för att synliggöra specifika delar av levande celler och vävnader med hjälp av fluorofores. En eller flera fluoforer tillsätts provet som ska undersökas. Då fluoroforesförberedda prover utsätts för så kallat excitationsljus så absorberas excitationsenergin av fluoroforerna och de börjar avge så kallat utstrålningsljus som man kan se med mikroskopet. Med denna teknik kan tumörceller urskiljas bland vanliga celler, biologiska processer kan synliggöras och följas samt så kan närvaro eller frånvaro av antikroppar urskiljas, för att nämna några applikationer.

Den utmärkta optiken och de utmärkta mekaniska delarna hos iScope fluorescence till ett av de bästa mikroskopen för dessa applikationer.

The iScope fluorescence microscopy can be supplied with:
• 6-position rotating attachment with 100 W mercury vapor light source
• 3-position slider attachment with 100 W mercury vapor light source
• 1-position slider attachment with a 3 W NeoLED 465-475 nm blue light source
• 1-position slider attachment with a 3 W NeoLED 520-530 nm green light source

EYEPIECE(S)
Extended EWF 10x/22 mm eyepieces, Ø 30 mm tubes. Eyepieces can be secured to the tubes

HEADS
Binocular and trinocular Siedentopf type heads are with 30° inclined tubes, interpupillary distance from 48 to 76 mm and is equipped with diopter adjustment(s). An unique rotating system allows the ergonomical positioning of both tubes in a high (431 mm) and in a low position (397 mm). The trinocular head comes with a Ø 23.2 mm photo port. An optional ergonomic titling head and corresponding Ø 23.2 mm photo tube attachment are also available (only infinity corrected models).
To order an iScope with tilting head use reference IS.3158-xxx

NOSEPIECE
Revolving and reversed nosepiece for maximum 5 objectives

STAGES
• 216 x 150 mm rackless stage equipped with double slide holder and integrated 79 x 52 mm mechanical stage. The iScope® rackless stage has no protruding parts, enabling more smooth movements and is safer
• 220 x 157 mm ceramic stage with integrated 75 x 50 mm mechanical stage protects with a very tough ceramic surface, against marks and scratches. The surface also ensures stray light not to be reflected from the stage into the objective

CONDENSER FOR BRIGHTFIELD
The standard height adjustable Abbe N.A. 1.25 condenser for brightfield accepts sliders with phase annuli suitable for plan phase 10x/20x or S40x/S100x oil immersion IOS objectives for economical phase contrast. With magnification indication. A slider with a simple dark stop for dark field up to 400x magnification is also available

CONDENSER FOR ZERNIKE PHASE CONTRAST
An optional height adjustable Zernike NA. 1.25 phase contrast disc condenser with plan phase 10/20/S40x and S100x oil immersion objectives and dark field stop position for dark field contrast up to 400x magnification

FOCUSING
Coaxial coarse and fine adjustments, 200 graduations, 1 μm per graduation, 200 μm per rotation, total travel approximately 24 mm. Supplied with an adjustable rack stop to prevent damage to sample and objectives. The coarse adjustments are equipped with friction control

ILLUMINATION
3 W adjustable Köhler NeoLED diascopic illumination with internal 100-240 V power supply
The innovative NeoLED design offer larger apertures, allowing the optical system of the iScope microscope to produce images at higher resolutions, very close to the theoretical diffraction limit of the optics. Other benefits of the NeoLED is the low energy consumption, no heating and a long operating lifetime
The Köhler illumination on models with IOS plan and plan phase infinity corrected system provides high contrast and a maximum achievable resolving power of the optics. Also available for other models as an option

ILLUMINATION FOR FLUORESCENCE
The 6-positions rotating or 3-positions slider fluorescence attachments are supplied with standard 100 W HBO mercury vapor light source for broad powerful light spectrum and 100-240 V external power supply with indication of the life time of 100 W HBO lamp.
Supplied with standard fluorescence filters sets for blue and green excitation and a UV protection shield
The iScope fluorescence microscopes equipped with a 100 W mercury vapor light source can be supplied with more specific, optimized and application related fluorescence filters sets
Two single position fluorescence attachments are supplied with 460-495 nm 3 W LED for blue excitation or 510-550 nm 3 W LED light source for green excitation and 100-240 Vac/7.5 Vdc external power supply. Supplied with standard fluorescence filters sets for blue and green excitation

ICARE SENSOR
The unique iCare Sensor is developed to avoid unnecessary loss of energy. The illumination of the microscope automatically switches off shortly after microscopists step away from their position

CSS - CABLE STORAGE SYSTEM
iScope allows users to easily insert the power cable into the backside of the instrument, which enables easy storage. The integrated carrying grip at the back of the microscope ensures safe transportation of the microscope

PACKAGING
Supplied with power cord, dust cover, a spare fuse, user manual and 5 ml immersion oil for models with S100x objective. Models with 100 W mercury vapor light source have a UV protection shield. All packed in polystyrene boxes